Publications
Preprints
Allen, M. C., Ritchie, M. W., El-Saadi, M. I., & MacMillan, H. A. (2023). Effects of a high cholesterol diet on Drosophila chill tolerance are highly context-dependent. 2023-09. DOI: https://doi.org/10.1101/2023.09.09.556984
Chalmer, S.C., Rudman, S.M., Andersen, M.K., Schmidt, P., MacMillan, H.A. Elemental stoichiometry and insect chill tolerance: evolved and plastic changes in organismal Na+ and K+ content in Drosophila. https://doi.org/10.1101/2022.08.18.504319
2023
El-Saadi, M.I., Brzezinski, K., Hinz, A., Phillips, L., Wong, A., Gerber, L., Overgaard, J., MacMillan, H.A. Locust gut epithelia do not become more permeable to fluorescent dextran and bacteria in the cold. Journal of Experimental Biology 226(16), jeb246306.
Ritchie, M.W., Cheslock, A., Bourdages, M.P.T., Hamilton, B.M., Provencher, J.F., Allison, J.E., MacMillan, H.A. (in press) Characterizing microplastic ingestion, transformation, and excretion in insects using fluorescent plastics. Conservation Physiology.
El Saadi, M.I., MacMillan, H.A., Ferguson, L.V. (2023) Cold immune activation in chill susceptible insects. Current Opinion in Insect Science 58: 101054.
Robertson, R.M., MacMillan, H.A., Andersen, M.K. (2023) A cold and quiet brain: mechanisms of insect CNS arrest at low temperatures. Current Opinion in Insect Science 58: 101055.
2022
Robichaud, J.A., Bulté, G., MacMillan, H.A., Cooke, S.J. (2022) Five months under ice: biologging reveals behavior patterns of overwintering freshwater turtles. Canadian Journal of Zoology.
Muzzatti, M.J., McConnell, E., Neave, S., MacMillan, H.A., Bertram, S.M. (2022) Fruitful female fecundity after feeding Gryllodes sigillatus (Orthoptera: Gryllidae) royal jelly. The Canadian Entomologist 154(1): E50.
Andersen, M.K., Robertson, R.M., MacMillan, H.A. (2022) Plasticity in Na+/K+-ATPase thermal kinetics drives variation in the critical thermal minimum of adult Drosophila melanogaster. The Journal of Experimental Biology 225: 244923.
Wettlaufer, J.D., Ye, A., MacMillan, H.A., Martin, J.D. (2022) A test of the competitive ability – cold tolerance trade-off hypothesis in seasonally breeding beetles. Ecological Entomology. een.13201.
Cooke, S.J., Hultine, K.R., Rummer, J.L., Fangue, N.A., Seebacher, F., Eliason, E.J., MacMillan, H.A., Fuller, A., Franklin, C.E. (2022) Elevating the impact of conservation physiology by building a community devoted to excellence, transparency, ethics, integrity, and mutual respect. Conservation Physiology 10(1): coac015.
Fudlosid, S., Ritchie, M.W. Muzzatti, M.J., Allison, J.E., Provencher, J., and MacMillan, H.A. (2022) Ingestion of microplastic fibres, but not microplastic beads, impacts growth rates in the tropical house cricket Gryllodes sigillatus. Frontiers in Physiology 13: 1-12.
2021
O’Neill, E., Davis, H.E., MacMillan, H.A. (2021) A lack of repeatability creates the illusion of a trade-off between basal and plastic cold tolerance. Proceedings of the Royal Society B: Biological Sciences 288(1964): 20212121.
Cheslock, A., Andersen, M.K., MacMillan, H.A. (2021) Thermal acclimation alters the roles of Na+/K+-ATPase activity in a tissue-specific manner in Drosophila melanogaster. Comparative Biochemistry and Physiology A 256: 1101934.
Davis H.E., Cheslock A., MacMillan H.A. (2021) Chill coma onset and recovery fail to reveal true variation in thermal performance among populations of Drosophila melanogaster. Scientific Reports 11: 10876.
Tremblay, P., MacMillan, H.A., Kharouba, H.M. (2021) Autumn larval cold tolerance does not predict the northern range limit of a widespread butterfly species. Ecology and Evolution 11: 8332–8346.
Ritchie, M.W., Dawson, J., MacMillan, H.A. (2021) A simple and dynamic thermal gradient device for measuring thermal performance in small ectotherms. Current Research in Insect Science.
Cooke et al. (2021) One hundred research questions in conservation physiology for generating actionable evidence to inform conservation policy and practice. Conservation Physiology 9: coab009.
2020
Carrington, J., Andersen, M.K., Brzezinski, K., MacMillan, H.A. (2020) Hyperkalemia, not apoptosis, accurately predicts chilling injury in individual locusts. Proceedings of the Royal Society B: Biological Sciences. 287: 20201663.
Brzezinski, K., MacMillan, H.A. (2020) Chilling induces unidirectional solute leak through the locust gut epithelia. The Journal of Experimental Biology 223: jeb215475.
El-Saadi, M.I., Ritchie, M.W., Davis, H.E., MacMillan, H.A. Warm periods in repeated cold stresses protect Drosophila against ionoregulatory collapse, chilling injury, and reproductive deficits. Journal of Insect Physiology 123: 104055.
Livingston, D.L.,Patel, H., Donini, A., MacMillan, H.A. (2020) Active transport of brilliant blue FCF across the Drosophila midgut and Malpighian tubule epithelia. Comparative Biochemistry and Physiology A: Molecular and Integrative Physiology 239: 110588.
Cooke, S.J., Madliger, C.L., Cramp, R.L., et al. (2020) Reframing conservation physiology to be more inclusive, integrative, relevant and forward-looking: reflections and a horizon scan. Conservation Physiology 8: coaa016.
2019
Jass, A., Yerushalmi, G.Y., Davis, H.E., Donini, A., MacMillan, H.A. (2019) An impressive capacity for cold tolerance plasticity protects against ionoregulatory collapse in the disease vector, Aedes aegypti. The Journal of Experimental Biology 222: jeb214056
MacMillan, H.A. (2019) Dissecting cause from consequence: A systematic approach to thermal limits (Invited). The Journal of Experimental Biology 222: jeb191593.
Kolosov, D., Donly, C., MacMillan, H.A., O'Donnell M.J. (2019) Transcriptomic analysis of the Malpighian tubule of Trichoplusia ni: clues to mechanisms for switching from ion secretion to ion reabsorption in the distal ileac plexus. Journal of Insect Physiology 112: 73-89.
2018
MacMillan, H. A., Nazal, B., Wali, S., Yerushalmi, G. Y., Misyura, L., Donini, A. and Paluzzi, J.-P. (2018). Anti-diuretic activity of a CAPA neuropeptide can compromise Drosophila chill tolerance. The Journal of Experimental Biology. 221: jeb.185884.
Yerushalmi, G., Misyura, L., MacMillan, H.A., Donini, A. (2018) Functional plasticity of the gut and Malpighian tubules underlies cold acclimation and mitigates cold-induced hyperkalemia in Drosophila melanogaster. The Journal of Experimental Biology. 221: jeb174904.
2017
Andersen, M.K., MacMillan, H.A., Donini, A., Overgaard, J. (2017) Cold tolerance of Drosophila species is tightly linked to epithelial K+ transport capacity of the Malpighian tubules and rectal pads. The Journal of Experimental Biology. 220: 4261-4269.
MacMillan H.A., Nørgård, M., MacLean H.J., Overgaard, J., Williams, C.J.A. (2017) A critical test of Drosophila anaesthetics: Isoflurane and sevoflurane are benign alternatives to cold and CO2. Journal of Insect Physiology. 101, 97-106.
MacMillan H.A., Yerushalmi, G., Jonusaite, S., Kelly, S.P., Donini, A. (2017) Thermal acclimation mitigates cold-induced paracellular leak from the Drosophila gut. Scientific Reports. 7, 8807.
Jørgensen, L.B., Overgaard, J., MacMillan, H.A. (2017) Paralysis and heart failure precede ion balance disruption in heat-stressed European green crabs. Journal of Thermal Biology. 68, 186-194.
O’Sullivan, J., MacMillan, H.A., Overgaard, J. (2017) Heat stress is associated with disruption of ion balance in the migratory locust. Journal of Thermal Biology. 68, 177-185.
Scarf I., Daniel A., MacMillan H.A., Katz N. (2017) The effect of fasting and body reserves on cold tolerance in two pit-building insect predators. Current Zoology. 63: 287-294.
Overgaard, J.O., MacMillan H.A. (2017) The integrative physiology of insect chill tolerance. Annual Review of Physiology. 79, 187-208.
Andersen, M. K., Folkersen, R., MacMillan, H.A., Overgaard, J. (2017) Cold acclimation improves chill tolerance in the migratory locust through preservation of ion balance and membrane potential. Journal of Experimental Biology. 220: 487-496.
2016
Yerushalmi, G.Y., Misyura, L., Donini, A., MacMillan, H.A. (2016) Chronic dietary salt stress mitigates hyperkalemia and facilitates chill coma recovery in Drosophila melanogaster. Journal of Insect Physiology. 95: 89-97.
Ollson, T., Malmendal, A., MacMillan, H.A., Nyberg, N., Stærk, D., Overgaard, J. (2016) Hemolymph metabolites and osmolality are tightly linked to cold tolerance of Drosophila species: a comparative study. The Journal of Experimental Biology. 219: 2504-2513.
MacMillan, H.A., Knee, J.M., Dennis, A.B., Udaka, H., Marshall, K.E., Merritt, T.J.S., Sinclair, B.J. (2016) Cold acclimation wholly reorganizes the Drosophila melanogaster transcriptome and metabolome. Scientific Reports. 6: 28999.
MacMillan, H.A., Schou, M.F., Kristensen, T.N., Overgaard, J. (2016). Preservation of potassium balance is strongly associated with insect cold tolerance in the field: A seasonal study of Drosophila subobscura. Biology Letters. 12: 20160123.
Scarf, I., Wexler, Y., MacMillan, H.A., Presman, S., Simson, E., Rosenstein, S. (2016) The negative effect of starvation and the positive effect of mild thermal stress on thermal tolerance of the red flour beetle. The Science of Nature. 103:20.
2015
MacMillan, H.A. Andersen, J.L., Davies, S.A., Overgaard, J. (2015) The capacity to maintain ion and water homeostasis underlies interspecific variation in Drosophila cold tolerance. Scientific Reports. 5, 18607.
MacMillan, H.A. Baatrup, E., Overgaard, J. (2015) Concurrent effects of cold and hyperkalemia cause insect chilling injury. Proceedings of the Royal Society B. 282, 20151483.
MacMillan, H.A., Andersen, J.L., Loeschcke, V., Overgaard, J. (2015) Sodium distribution predicts the chill tolerance of Drosophila melanogaster raised in different thermal conditions. American Journal of Physiology: Regulatory, Integrative, and Comparative Physiology. 308, 823-831.
Andersen, J.L., MacMillan, H.A., Overgaard, J. (2015) Muscle membrane potential and insect chill coma. The Journal of Experimental Biology. 218, 2492-2495.
Andersen, J.L., MacMillan, H.A., Overgaard, J. (2015) Temperate Drosophila perserve cardiac function at low temperature. Journal of Insect Physiology. 77, 26-32.
Andersen J.L., Manenti, T., Sørensen, J.G., MacMillan, H.A., Loeschcke, V., & Overgaard, J. (2015) How to assess Drosophila cold tolerance: chill coma temperature and lower lethal temperature are the best predictors of cold distribution limits. Functional Ecology. 29, 55-65.
Shamchuk A.L., MacMillan H.A.. (2015) Crossing boundaries and building bridges: integrative zoology. Canadian Journal of Zoology. 93: 677–678.
Coello Alvarado, L.E., MacMillan, H.A., Sinclair, B.J. (2015) Chill-tolerant Gryllus crickets maintain ion balance at low temperatures. Journal of Insect Physiology. 77, 15-25
MacMillan, H.A., Ferguson, L.V., Nicolai, A., Donini, A., Staples, J.F., Sinclair, B.J. (2015) Parallel ionoregulatory adjustments underlie phenotypic plasticity and evolution of Drosophila cold tolerance. The Journal of Experimental Biology. 218, 423-432.
2014
MacMillan, H.A., Findsen, A., Pedersen, T.H. & Overgaard, J. (2014) Cold-induced depolarization of insect muscle: Differing roles of extracellular K+ during acute and chronic chilling. The Journal of Experimental Biology. 217, 2930-2938.
MacMillan, H.A. & Hughson, B.N. (2014) A high-throughput method of hemolymph extraction from adult Drosophila without anaesthesia. Journal of Insect Physiology. 63, 27-31.
2013
Lake, S. L., MacMillan, H. A., Williams, C. M., & Sinclair, B. J. (2013). Static and dynamic approaches yield similar estimates of the thermal sensitivity of insect metabolism. Journal of Insect Physiology, 59(8), 761–766.
Sinclair, B. J., Ferguson, L. V., Salehipour-shirazi, G., & MacMillan, H. A. (2013). Cross-tolerance and cross-talk in the cold: Relating low temperatures to desiccation and immune stress in Iinsects. Integrative and Comparative Biology. 53(4), 546-556.
Sinclair, B. J., Stinziano, J., Williams, C. M., MacMillan, H. A., Marshall, K., & Storey, K. (2013). Real-time measurement of metabolic rate during freezing and thawing of the wood frog, Rana sylvatica: implications for overwinter energy use. The Journal of Experimental Biology, 216, 292–302.
2012
MacMillan, H. A., Williams, C. M., Staples, J. F., & Sinclair, B. J. (2012). Reestablishment of ion homeostasis during chill-coma recovery in the cricket Gryllus pennsylvanicus. Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20750–20755.
MacMillan, H. A., Williams, C. M., Staples, J. F., & Sinclair, B. J. (2012). Metabolism and energy supply below the critical thermal minimum of a chill-susceptible insect. The Journal of Experimental Biology, 215(Pt 8), 1366–1372.
Williams, C. M., Marshall, K. E., MacMillan, H. A., Dzurisin, J. D. K., Hellmann, J. J., & Sinclair, B. J. (2012). Thermal variability increases the impact of autumnal warming and drives metabolic depression in an overwintering butterfly. PLoS one, 7(3), e34470.
2011
Ransberry, V. E., MacMillan, H. A., & Sinclair, B. J. (2011). The relationship between chill-coma onset and recovery at the extremes of the thermal window of Drosophila melanogaster. Physiological and Biochemical Zoology, 84(6), 553–559.
MacMillan, H. A., & Sinclair, B. J. (2011). Mechanisms underlying insect chill-coma. Journal of Insect Physiology, 57(1), 12–20.
MacMillan, H. A., & Sinclair, B. J. (2011). The role of the gut in insect chilling injury: cold-induced disruption of osmoregulation in the fall field cricket, Gryllus pennsylvanicus. The Journal of Experimental Biology, 214, 726–734.
Williams, C. M., Thomas, R. H., MacMillan, H. A., Marshall, K. E., & Sinclair, B. J. (2011). Triacylglyceride measurement in small quantities of homogenised insect tissue: comparisons and caveats. Journal of Insect Physiology, 57(12), 1602–13.
2010
Bazinet, A. L., Marshall, K. E., MacMillan, H. A., Williams, C. M., & Sinclair, B. J. (2010). Rapid changes in desiccation resistance in Drosophila melanogaster are facilitated by changes in cuticular permeability. Journal of insect physiology, 56(12), 2006–12.
2009
MacMillan, H. A., Guglielmo, C. G., & Sinclair, B. J. (2009). Membrane remodeling and glucose in Drosophila melanogaster: a test of rapid cold-hardening and chilling tolerance hypotheses. Journal of Insect Physiology, 55(3), 243–9.
MacMillan, H. A., Walsh, J. P., & Sinclair, B. J. (2009). The effects of selection for cold tolerance on cross-tolerance to other environmental stressors in Drosophila melanogaster. Insect Science, 16(3), 263–276.
